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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in lots of tumors, which results in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical function in advertising cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two further splice variants were described but specific nucleotide sequence data is not nevertheless offered.
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Blocking peptides are peptides that bind specifically to your concentrate on antibody and block antibody binding. These peptide commonly contains the epitope recognized through the antibody.
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This guide outlines further more probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This manual outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
Antibodies bound to the blocking peptide no more bind on the epitope within the concentrate on protein. By evaluating the staining through the blocked antibody versus the antibody alone, you can see which staining is distinct.
Focus on Facts Cyclin E belongs towards the extremely conserved cyclin family members, whose customers are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which add to the temporal coordination of each and every mitotic party. Cyclin E types a fancy with and capabilities for a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates with the G1-S phase boundary which is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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